Table 4.
454 sequencing of plasma virus in macaque M03250
| Mutation | Percent mutant |
|||
|---|---|---|---|---|
| Assay background | Inoculum | Week 13 | Week 24 | |
| K65Ra | 0.17 | 0.17 | 0.28 | 4.42 |
| L100Ia | 0.00 | 0.00 | 0.02 | 1.36 |
| K101Ea | 0.12 | 0.05 | 0.27 | 0.04 |
| K103N (AAC)a | 0.00 | 0.00 | 0.00 | 78.4 |
| K103N (AAT)a | 0.02 | 0.00 | 0.00 | 0.2 |
| Y181Cb | 0.00 | 0.09 | 0.05 | 0.56 |
| M184Ib | 0.06 | 0.11 | 0.34 | 60.6 |
| M184Vb | 0.00 | 0.15 | 0.66 | 0.6 |
| Y188Cb | 0.00 | 0.14 | 0.58 | 0.45 |
| G190Ab | 0.00 | 0.00 | 0.00 | 0.4 |
a
Amplicon 1 comprised 265 bp encoding amino acids 41 through 103. The total numbers of sequences obtained for this amplicon were 58,431 for the assay background, 10,836 for the inoculum, 12,130 for the week 13 plasma sample, and 36,544 for the week 24 plasma sample.
b
Amplicon 2 comprised 160 bp encoding amino acids 181 through 219. The total numbers of sequences obtained for this amplicon were 45,385 for the assay background, 25,831 for the inoculum, 24,636 for the week 13 plasma sample, and 49,236 for the week 24 plasma sample.