Fig 1.

Sequence alignment of the AAPs from AAV serotypes 1 to 13. An annotated multiple-sequence alignment of the AAP sequences of 13 AAV serotypes is shown. Sequences are visualized according to the ClustalX coloring scheme. Colored residues are evolutionarily conserved, and the coloring reflects the physicochemical properties of the residues. Regions of interest are annotated above the alignment. Inverted triangles denote the residues experimentally mutated in this study. Predicted secondary structural (SS) elements (strand, helix) for the sequence and amino acid numbering for the AAV serotype 2 AAP are displayed below the alignments.