Fig 4.

Modified MVA vectors elicit enhanced titers of HIV Env-specific antibodies in immunized macaques. (A) Titers of antibodies against vaccine-encoded gp120 were determined by ELISAs, at the indicated times, in macaques that were immunized with either the high-dose (2 × 10⁸ PFU) or low-dose (1 × 10⁷ PFU) MVAΔ4-HIV, MVAΔ5-HIV, or MVA-HIV vector. Symbols represent individual macaques, horizontal lines denote geometric mean titers, arrows indicate immunizations at weeks 0 and 9, and an asterisk denotes a P value of ≤0.05 (determined by a Kruskal-Wallis test). (B) Week 10 sera from macaques immunized with high-dose MVAΔ4-HIV (red) or MVA-HIV (blue) were assayed at a 1:20 dilution for ex vivo neutralization activity against an HIV Con-C Env-pseudotyped lentivirus reporter, as described in the supplemental material. Data are normalized to individual macaques' preimmune values. Horizontal lines denote group means. NS indicates “not significant.”