Fig 2.

(A) Snapshot of a terminase phylogeny tree of 109 TerL amino acid sequences and P70 TerL computed by using the neighbor-joining method from a ClustalW alignment. Numbers at nodes indicate bootstrap values. The P70 TerL sequence is highlighted, other Listeria phage TerL sequences are indicated by black dots. (B) Restriction profile analysis of P70 genome structure. (Panel 1) Restriction digest of purified P70 DNA with nine different restriction endonucleases. Lanes c to g and j to m: KpnI, ClaI, SwaI, SalI, EcoRI, EcoRV, PacI, NdeI, and HindIII. Lanes a, b, h, i, n, and o: 1-kb and Lambda mix markers (Fermentas). (Panel 2) PFGE run of P70 DNA (lane a). Lanes b and c: midrange PFGE markers I and II (New England BioLabs). Numbers indicate marker band sizes in kilobases.