Table 1.
BAC recombineering, sequencing, and PCR oligonucleotides
Oligonucleotide name | Sequence (5′ to 3′) | Length (no. of bases) |
---|---|---|
gDgalKf | CCCGGCAGCGTGCCTCACCTCGAAGGCCTACCAACAGGGCGTGACGGTCGCCTGTTGACAATTAATCATCGGCA | 74 |
gDgalKr | GGCGATTTTTAAGCTGTATAGGGCGACGGTGCGCTGGTTTTCGGGGATAATCAGCACTGTCCTGCTCCTT | 70 |
D215G/R222N/F223If | CCCGGCAGCGTGCCTCACCTCGAAGGCCTACCAACAGGGCGTGACGGTCGGCAGCATCGGGATGCTCCCCAACATTATCCCCGAAAACCAGCGCACCGTCGCCCTATACAGCTTAAAAATCGCC | 124 |
D215G/R222N/F223Ir | GGCGATTTTTAAGCTGTATAGGGCGACGGTGCGCTGGTTTTCGGGGATAATGTTGGGGAGCATCCCGATGCTGCCGACCGTCACGCCCTGTTGGTAGGCCTTCGAGGTGAGGCACGCTGCCGGG | 124 |
gDF101 | GCCCCGCTGGAGCTACTATGACA | 23 |
gDF103 | CATAAGCTGCATTGCGAACGACTA | 24 |
gDF106 | GGACGAGGCCCGAAAGCACAC | 21 |
gDR101 | TCGACGGGATGTGCCAGTTTG | 21 |
gDR103 | CCACACGGGCCCAGAGGTA | 19 |
gDR106 | TTCGGGGATAAAGCGGGGTAG | 21 |
NotIF1 | GTCGCGGCCGCCACCATGGAGCCTCCTGGAGACTGG | 34 |
XhoIR2 | AGTCTCGAGGTCAGTGGTTTGGGCTCCTCCCCGTG | 33 |
sHveAF1 | GTCGCGGCCGCCACCATGGAGCCTCCTGGAGGTTGG | 36 |
sHveAR2 | AGTCTCGAGGTCAGCTCCTCCCAGTGAATGCGGG | 34 |
The underlined nucleotides indicate the sites of mutations.