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. 2012 Dec;86(23):12770–12778. doi: 10.1128/JVI.01545-12

Fig 5.

Fig 5

The secretion of chemokines, interferon-stimulated proteins, and endogenous danger signal proteins is increased by IFN-β priming from HSV-1-infected macrophages. Macrophages were left untreated (control [CTR]) or primed with 200 IU/ml of IFN-β for 4 h, after which they were infected with HSV-1 (MOI, 1) for 18 h. Cell supernatants were collected, and cell lysates were prepared at 18 hpi. (A) Luminex analysis of unconcentrated cell supernatants for selected chemokines from control (0), IFN-β-primed, HSV-1-infected, and IFN-β-primed plus HSV-1-infected macrophages. The assay was done for the following chemokines: CCL2, CCL3, CCL4, and CCL5. (B) Western blot analysis from macrophage total cell lysates and concentrated cell supernatants for interferon-stimulated proteins IFIT2, IFIT3, STAT1 p84/p91, and MxA. (C) Western blot analysis from macrophage total cell lysates and concentrated cell supernatants for endogenous danger signal proteins HSP27, HSP90, S100-A9, and galectin-3.