(Continued from facing page.)
Photographs showing development of single-node cuttings of potato
plants grown in vitro on various media after 10 d of culture. Each
photograph, except D, shows one to five representative cuttings for a
culture condition. A, Tuber-inducing treatment with 8% Suc in the
nutrient medium. B, Noninducing treatment with 1% Suc. C, Noninducing
treatment with 8% Suc plus 0.5 μm GA4/7. D,
A series of Suc concentrations: 8, 6, 4, 2, and 1% from left to right.
E, 8% Suc plus 0.01 μm GA4/7. F, 8% Suc
plus 0.03 μm GA4/7. G, 8% Suc plus 0.1
μm GA4/7. H, 8% Suc plus 0.3
μm GA4/7. I, 8% Suc plus 1.0
μm GA4/7. J, Formation of secondary stolons
after transfer of the cuttings from 8% Suc medium to 8% Suc plus
GA4/7 medium at d 5 (the bottom cutting) or at d 10 (the
two top cuttings) and further culture for 5 d. K, Formation of
secondary tubers by transferring cuttings first from 8% Suc medium to
8% Suc plus GA4/7 medium at d 5 and then back to 8% Suc
medium at d 10 and further culturing for 5 d. The short arrow
points to the first tuber and the long arrow points to a secondary one.
L, 8% Suc plus ABA. M, 8% Suc plus IAA. N, 1% Suc plus ABA; arrow
indicates the short stolon. O, 1% Suc plus IAA. P, 8% Suc plus
GA4/7 plus ABA. Q, 8% Suc plus GA4/7 plus IAA.
R, 8% Suc (top line) and 8% Suc plus BA (bottom line).