Table 1.
Biosynthesis of various odd-carbon chemicals by different pathway modulation schemes
| Product titers (mg/L) ± SD |
||||||
| Prop |
3HV |
T2P |
Val |
PenOH |
||
| Pathways (substrate*→product) | B | C | D | E | F | Strains† |
| A′→B | 804 ± 20 | ND | ND | ND | ND | Pal25 |
| A→C (S-3HV) | 35 ± 20 | 312 ± 6 | ND | ND | ND | (18) |
| A′→C (R-3HV) | 140 ± 8 | 963 ± 68 | ND | ND | ND | (18) |
| A′→E | 110 ± 10 | ND | ND | 398 ± 15 | ND | Pal24 |
| A′→F | 78 ± 6 | ND | ND | 81 ± 6 | 116 ± 14 | Pal23 |
| B→C (S-3HV)‡ | 2,051 ± 67 | ND | ND | ND | (18) | |
| B→C (R-3HV) ‡ | 1,964 ± 99 | ND | ND | ND | (18) | |
| B→D‡ | 869 ± 54 | 57 ± 2 | ND | ND | Pal3 | |
| B→E‡ | ND | ND | 554 ± 28 | ND | Pal12 | |
| B→F‡ | ND | ND | 360 ± 23 | 358 ± 21 | Pal11 | |
| D→F§ | ND | ND | 799 ± 33 | 1,317 ± 45 | BL12 | |
| E→F§ | ND | ND | ND | 530 ± 18 | BL4 | |
A, glucose; A′, glycerol; B, propionate (Prop); C, 3-hydroxyvalerate (3HV); D, trans-2-pentenoate (T2P); E, valerate (Val); F, pentanol (PenOH); ND, not detected.
*Feed amount: Glucose or glycerol (10 g/L); Prop (20 mM); T2P (2 g/L); Val (20 mM).
†Table S2.
‡Recombinant strains containing synthetic pathways that start with B were grown in media supplemented with 10 g/L of glucose in additional to propionate.
§Nearly all consumed substrate was converted (∼100 mol%) to products listed above.