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. 2012 Oct 15;109(44):17936–17941. doi: 10.1073/pnas.1216201109

Fig. 6.

Fig. 6.

Activities of PI3P binding domains. R-SNARE RPLs and 3Q-SNARE RPLs bearing Vam7tm,∆N were incubated with diC8-PI3P (47 μM) and control buffer or 1.1 μM of PX domain from Vam7p (Vam7(1-125)p), PX domain from SNX3, or FYVE domain from Hrs. The N-terminal GST tag of these PI3P binding domains had been precleaved with thrombin.