Fig. 4.
Asp184 and Asp189 are important for Spindlin1’s H3K4me3-binding activity. (A) ITC measurements of binding affinities between the wild-type, D184A, D184R, D189A, and D189R mutants of Spindlin1 and a 10-residue H3K4me3 peptide (aa 1–10). Kd values with fitting errors are indicated. (B) RT-PCR detection of prerRNA expression in cells expressing the wild-type and Asp184 and Asp189 mutants of Spindlin1 (Right). The expression levels of the wild-type and mutant Flag-Spindlin1 mRNA were also determined by RT-PCR (Left). Error bars represent the SD calculated from three independent experiment repeats. ***P < 0.002; **P < 0.01; and #P > 0.1.