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. 2012 Oct 17;109(44):18192–18197. doi: 10.1073/pnas.1209174109

Fig. 2.

Fig. 2.

Determination of epithelial permeability in pluteus larvae in vivo using FITC dextran conjugates of varying molecular weights and confocal microscopy. (A) Transmission image of pluteus larva; (B) confocal image of larva in a similar position as in A exposed to 4-kDa FITC dextran in seawater for 60 min; the green color in the extracellular matrix (ECM) indicates equilibration of FITC dextran between surrounding seawater (SW) and ECM; (C) confocal image of larva exposed to 40 kDa for 60 min; the dark color indicates lack of equilibration of FITC dextran between ECM and SW. (D) Equilibration of FITC dextrans of varying molecular weights between SW and ECM following 15- and 60-min incubation. Equilibration is represented as the ratio of FITC fluorescence within the ECM and the SW surrounding the larva. Low values indicate a low permeability. (E) Corresponding confocal (FM1-43-stained) and transmission images of the pluteus larva outer epithelium. Bars represent ±SD; n = 9.