Fig. 2.

Biotin complements the ∆FTN_0818 growth defect in minimal media. (A) WT and ΔFTN_0818 were grown in CHB with or without biotin and the OD₆₀₀ was measured every hour. (B) The concentration of biotinylated proteins in whole cell lysates of all strains was quantified after immunoprecipitation with anti-biotin antibodies. *P < 0.05; **P < 0.001. (C) Schematic of the biotin biosynthesis pathway with the proposed placement of FTN_0818 (steps before the generation of pimelate have not been defined in Francisella). (D) WT and ΔFTN_0818 were grown in CHB with or without pimelate and the OD₆₀₀ was measured every hour.