Fig. 2.

BapE is induced after prolonged DNA damage but does not affect DNA repair. (A) Mutation frequency (fold increase) of wild-type and bapE-ssrA strains after MMC treatment. Error bars indicate SD. Asterisk denotes significant difference between MMC-treated wild-type and bapE-ssrA mutant strains (P = 0.04) (B) Dynamics of SOS-induced gene induction as measured by qRT-PCR. Fold induction of each gene as a function of time after the addition of MMC. Error bars represent SD. (C) The endogenous levels of BapE and RecA protein were determined by Western blotting after 0, 1, 2, 4, and 8 h of MMC treatment (Inset shows blot and plot shows fold induction).