Fig. 4.

CASPR2 KD acts cell-autonomously in impairing synaptic network activity. (A) Representative images of mCherry-expressing transfected neurons. (B) Sample traces (Left) and amplitude summary graphs (Right) of NMDA-R–mediated EPSCs evoked in neurons transfected with control mCherry-expressing vector (Control) or mCherry expression vector coexpressing the CASPR2 shRNA without (CASPR2 KD) or with a CASPR2 rescue cDNA (KD + Rescue). (C and D) Membrane capacitance (C) and input resistance (D) recorded from neurons described in B. (E–G) Sample traces of mEPSCs (E), cumulative plot of mEPSC interevent intervals (Inset, summary graph of event frequency) (F), and summary graph of averaged mEPSC amplitudes (G) recorded from neurons described in B. (H–J) Same as E–G, but for mIPSCs. Data shown are means ± SEMs; number of cells/independent cultures analyzed are depicted in the bars. Statistical significance was evaluated by Student’s t test (*P < 0.05; **P < 0.01) except for the cumulative probabilities that were analyzed by the Kolmogorov–Smirnov test (***P < 0.001).