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. 2012 Oct 16;109(44):18180–18185. doi: 10.1073/pnas.1207977109

Fig. 1.

Fig. 1.

TtMscS Is an anion-selective MS channel. (A) Left: Single-channel traces recorded by a patch-clamp system from giant spheroplasts containing TtMscS (Upper Left) or EcMscS (Lower Left) at +20 mV. The number under the trace indicates the pressure applied to the patch during the event. Right: I-V curves for TtMscS and EcMscS channels at a 1:3 salt gradient (200 mM/600 mM); an ideal anion-selective channel with Erev = −27.7 mV according to the Nernst equation is indicated. The reversal potentials for TtMscS and EcMscS are −12 ± 1.5 mV (n = 3 patches) and −2 ± 1.3 mV (n = 3 patches), respectively. (B) Left: Single-channel open and closed traces recorded by a patch-clamp system from giant liposomes containing TtMscS (Upper Left) or EcMscS (Lower Left) at +50 mV. The number under the trace indicates the pressure applied to the patch during the event. Right: I-V curves for TtMscS and EcMscS channels at a 10:1 KCl gradient (150 mM/15 mM); an ideal anion-selective channel with Erev = −58 mV according to the Nernst equation is indicated. The reversal potentials for TtMscS and EcMscS are −39 ± 1.7 mV (n = 4 patches) and −4 ± 1.5 mV (n = 4 patches), respectively.