Fig. 1.
GFP structure and thermal folding. (A) Ribbon diagram of Citrine (Yellow version of GFP, PDB ID: 1HUY). Residues marked in orange, green and mauve are Lys3, Asn198, and Asn212, respectively. (B) Splay representation of GFP. The N-terminus β-strands are represented in blue, the kinked helix at the center of the β-strand barrel is in green, the three β-strands in the center, which form local contacts are in silver, and the C-terminus β-strands are in red. (C) Fraction of GFP in the NBA, INT, and UBA as a function of temperature, T, are shown in triangles, diamonds, and circles respectively. The left inset shows fINT(T) as a function of T. The right inset shows energy, E, and the heat capacity, Cv as a function of T. (D) Free energy of GFP as a function of E for temperatures above and below the melting temperature (T = 379 K). The structure of one of the intermediates is shown in splay representation.