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A three-state model that accounts for hierarchical (β < 1) folding of FRET-PGK and is used to validate the effective two-state model. The states N, I, and U have a linear dependence of free energy on the reaction coordinate j (gray slope) and temperature (arrow indicating tilt) given by the formula ΔG(φ) = G(1)φ(T - Tm). The free energy barriers are referenced to the average free energy of neighboring states as illustrated for 
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