Table I.
Regulation of βGLU, CHN, and βGLU-promoter activity by auxin, cytokinin, and ABA in cultured leaf discs
| Culture Mediuma | βGLU
|
CHN
|
GUS
|
|||
|---|---|---|---|---|---|---|
| Control | ABAb | Control | ABA | Control | ABA | |
| μg equivalents g−1 fresh wtc | pmol methylumbelliferol min−1g−1fresh wt | |||||
| −Auxin, −Cytokinin | 95.9 ± 5.4d | 8.4 ± 3.2 | 115 ± 10.7 | 123 ± 11.4 | 58.9 ± 7.4 | 7.1 ± 2.8 |
| +Auxin, −Cytokinin | 56.4 ± 6.1 | 0.7 ± 0.3 | 62.0 ± 7.4 | 67.6 ± 4.2 | 34.6 ± 5.8 | 3.8 ± 2.1 |
| −Auxin, +Cytokinin | 47.6 ± 5.4 | 2.1 ± 1.4 | 59.7 ± 6.2 | 62.3 ± 7.1 | 24.7 ± 6.4 | 4.2 ± 3.7 |
| +Auxin, +Cytokinin | 10.5 ± 3.2 | 0.2 ± 0.1 | 12.4 ± 2.8 | 10.1 ± 3.4 | 7.1 ± 2.4 | 0.5 ± 0.5 |
a
Discs explanted from leaves of the Glb-GUS transformant were incubated for 4 d on S0 medium supplemented as indicated with auxin (11 μm NAA) and cytokinin (1.4 μm kinetin).
b
10 μm final concentration.
c
Enzyme activity is expressed as microgram equivalents of the class I isoforms.
d
Mean values ± se for 12 replicate samples.