Fig. 2.

The effect of the five individual mutations on the kinetics of R16 and R17 compared to the wild-type proteins and core-swapped domains with all five substitutions. (A) Chevron plots of the conservative R16 mutants that have only a small effect on the kinetics. (B) Chevron plots of the two R16 mutants that have a more significant effect on the kinetics: E18F , K25V, and the double mutant E18F/K25V. (C) Chevron plots of the mutations made in B made in an R17 background. Wild-type data are taken from Scott et al. (6).