Effect of ibuprofen, PLGA NPs and ibuprofen-loaded PLGA NPs on cell proliferation.
Notes: MKN-45 cells (5 × 104 cells/well) were seeded in 24-well plates and incubated overnight. The cells were then treated with ibuprofen, ibuprofen-loaded PLGA NPs, or PLGA NPs for 24 hours. The cells were harvested and counted. Viability was determined by Trypan blue dye exclusion. Cell growth was calculated by averaging cell counts and as expressed as the percentage of the control, represented by MKN-45 cells grown in culture medium alone or culture medium with DMSO (100%). The mean values from three independent experiments performed in duplicate are presented. Ibuprofen significantly inhibited the proliferation of MKN-45 cells only in cells treated with ibuprofen-loaded PLGA NPs. Bars, SD; *P < 0.5; †P < 0.005; Student’s t-test.
Abbreviations: PLGA, poly(lactic-co-glycolic acid); NPs, nanoparticles; DMSO, dimethyl sulfoxide.