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Real-time PCR analysis.
Notes: Analysis of f ANGPTL4 mRNA levels in MKN-45 cells treated with free ibuprofen (200 and 800 μM) or ibuprofen-loaded NPs for 2 hours by Real-Time PCR. Each sample was normalized against human GAPDH. Bars indicate the fold change values versus untreated MKN-45 cells of three independent experiments performed in triplicate. Bars, SD; *P < 0.005; Student’s t-test.
Abbreviations: NPs, nanoparticles; GAPDH, human glyceraldehyde 3-phosphate dehydrogenase.
