Fig. 4.

CCN5 is expressed in E6.5 embryos (early stages of egg cylinder differentiation). a 5 μm frozen sagittal sections of embryo were processed as described in Materials and Methods. A = Amnion; AC = Amniotic Cavity; C = Chorion; ECC = Exocoelomic Cavity; Ect = Ectoderm; EPC = Ectoplacental Cavity; End = Endoderm. b Zoom of black box from (a) showing germ layers. Scale Bars = 50 μm (a), 25 μm (b). CCN5 immunoreactivity is indicated by brown staining. Nuclei are shown by hematoxylin (blue) stain