Fig. 5.

CCN5 is expressed in E7.5 embryos (gastrulation complete). a 5 μm frozen sagittal sections of embryo were processed as described in Materials and Methods. A = Amnion; AC = Amniotic Cavity; C = Chorion; ECC = Exocoelomic Cavity; Ect = Ectoderm; End = Endoderm; EPC = Ectoplacental Cavity; Mes = Mesoderm. b Zoom of left black box from (a) showing germ layers. c Zoom of right black box from (a) showing germ layers. Scale Bars = 50 μm (a), 10 μm (b and c). CCN5 immunoreactivity is indicated by brown staining. Nuclei are shown by hematoxylin (blue) staining