Fig. 2.

Monitoring of change of bacterial population by PCR. Total genomic DNAs were isolated from the zeroth, third, fifth, seventh, and ninth successive cultivations started from pooled culture and used as the template for PCR. The successive cultivation was performed without any solvents (a), with n-hexnae (10 %, v/v) (b), and with n-hexane/cyclohexane mixture (1:1, 2 %, v/v) (c). The reference PCR products corresponded to the strains are shown in (d)