FIGURE 1.

[Zn²⁺]_i in MIN6 cultures exposed to Zn²⁺, H₂O₂, and Ca²⁺ channel antagonists (Expt. 6). MIN6 cultures were loaded with 5 μmol/L FluoZin3 for 30 min and the cells were washed (basal) and then exposed as indicated. The effects of a depolarizing Zn²⁺ exposure on [Zn²⁺]_i were determined, as was the antagonism of nimodipine. The effects of a nondepolarizing Zn²⁺ exposure on [Zn²⁺]_i was determined, as was the antagonism of mibefradil. The effects of 100 μmol/L H₂O₂ on [Zn²⁺]_i was determined. These experiments are from at least 3 independent experiments, n = 45–60 cells/condition. *Different from basal levels; ^#different from a depolarizing Zn²⁺ exposure; ^$different from a nondepolarizing Zn²⁺ exposure, P < 0.05. [Zn²⁺]_i, intracellular zinc concentration.