FIGURE 2.

ΜΙΝ6 cell death in cultures exposed to Zn²⁺ (A), GD (B), streptozotocin (C), or cytokines (D) and treated with therapeutic compounds (Expts. 1 and 2). MIN6 cultures were exposed to 35 μmol/L Zn²⁺ (A) or GD at normal density (B) and 7.5 mmol/L STZ (C) or mixed cytokines at high density (D) and the therapeutic compounds were present as indicated. Nim/Mib indicates the presence of 10 μmol/L each of nimodipine and mibefradil. Cell survival was assayed by MTT or propidium iodide staining (data not shown) after 24 h (A,B) and by MTT staining after 6 h (C,D) from at least 3 independent experiments. Cell viability was scaled to control untreated cultures (100% viability) after subtraction of the signal associated with near-complete death produced by exposure to 20 μmol/L A23187 for 24 h = 0 (mean ± SEM, n = 8–20). *Different from respective toxin alone at P < 0.05. 3-AP, 3-acetylpyridine; GD, glucose deprivation; Glu, glucose; lac, lactate; MTT, 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide; N, nicotinamide; Naph, 2-hydroxynaphthaldehyde; Nimo/Mib, nimodipine/mibefradil; P, pyruvate; R, resveratrol; S, sirtinol; STZ, streptozotocin; TPEN, N,N,N'N’-tetrakis(-)[2-pyridylmethyl]-ethylenediamine.