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. 2012 Sep 26;68(Pt 10):1191–1194. doi: 10.1107/S1744309112034197

Table 2. Crystallization conditions.

Method Hanging-drop vapour diffusion
Plate type Iwaki 24-well plate
Temperature (K) 277
Protein concentration (mg ml−1) 5
Buffer composition of protein solution 0.02 M Tris–HCl pH 8.0, 0.02 M NaCl
Composition of reservoir solution 22%(w/v) PEG 3350, 0.1 M citric acid pH 3.5
Volume and ratio of drop 2 µl protein and 2 µl reservoir, 1:1
Volume of reservoir (µl) 750