Method |
Sitting drop |
Sitting drop |
Sitting drop |
Plate type |
96-well Greiner |
96-well Greiner |
96-well Greiner |
Temperature (K) |
294 |
294 |
294 |
Protein concentration (mg ml−1) |
30 |
30 |
30 |
Buffer composition of protein solution |
20 mM HEPES pH 7.4, 0.15 M NaCl |
20 mM HEPES pH 7.4, 0.15 M NaCl |
20 mM HEPES pH 7.4, 0.15 M NaCl |
Composition of reservoir solution |
20% PEG 3350, 0.2 M NaCl |
20% PEG 3350, 0.1 M bis-Tris propane pH 6.5, 0.2 M KNO3
|
8% PEG 6000, 60 mM HEPES pH 7.0, 40 mM MES pH 6.0 |
Volume (nl) and ratio of drop |
100, 1:1 |
100, 1:1 |
100, 1:1 |
Volume of reservoir (µl) |
100 |
100 |
100 |