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. 2012 Sep 26;68(Pt 10):1209–1213. doi: 10.1107/S1744309112035294

Table 1. Crystallization conditions.

  Form I (SeMet) Form II (native) Form III (native)
Method Sitting drop Sitting drop Sitting drop
Plate type 96-well Greiner 96-well Greiner 96-well Greiner
Temperature (K) 294 294 294
Protein concentration (mg ml−1) 30 30 30
Buffer composition of protein solution 20 mM HEPES pH 7.4, 0.15 M NaCl 20 mM HEPES pH 7.4, 0.15 M NaCl 20 mM HEPES pH 7.4, 0.15 M NaCl
Composition of reservoir solution 20% PEG 3350, 0.2 M NaCl 20% PEG 3350, 0.1 M bis-Tris propane pH 6.5, 0.2 M KNO3 8% PEG 6000, 60 mM HEPES pH 7.0, 40 mM MES pH 6.0
Volume (nl) and ratio of drop 100, 1:1 100, 1:1 100, 1:1
Volume of reservoir (µl) 100 100 100