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Construct design and schematic representation of the Drp1 GG fusion protein. (a) Full-length Drp1 consists of an N-terminal GTPase domain (green) and a helical middle domain (blue) followed by an unstructured region (insert B or variable domain) and a C-terminal GED (pink). (b) The GG fusion protein consists of the complete GTPase domain (amino acids 1–327) fused to a C-terminal fragment of the GED (amino acids 711–736) via a (GS)4 linker.
