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. 2012 Sep 26;68(Pt 10):1217–1221. doi: 10.1107/S174430911203607X

Figure 2.

Figure 2

Purification of the Drp1 GG fusion protein. (a) Size-exclusion analysis. After Ni–NTA purification, proteins were loaded onto a Superdex 75 gel-filtration column and fractions containing protein were collected. In the absence of nucleotides, the protein eluted as a monomer at a retention volume of approximately 11 ml, which corresponds to a molecular mass of approximately 40 kDa. The retention volumes of molecular-mass standards (GE Healthcare) are displayed at the top. (b) SDS–PAGE analysis. Protein fractions were loaded onto a 15% SDS–PAGE gel and visualized by Coomassie Brilliant Blue staining. Lane E, eluted protein; lane M, protein molecular-mass markers (labelled in kDa on the left). The retention volumes are indicated at the top.