Table 2. Crystallization.

Method	Hanging drop
Plate type	Qiagen EasyXtal 24-well plates
Temperature (K)	294 ± 2
Protein concentration (mg ml−1)	1–6
Buffer composition of protein solution	10 mM Tris–HCl pH 7.5, 200 mM NaCl, 1 mM EDTA, 1 mM DTT
Composition of reservoir solution	0.1 M HEPES pH 8.0, 21% PEG MME 2000, 125 mM serine or 0.1 M potassium citrate, 20% PEG 3350, 125 mM serine
Volume (µl) and ratio of drop	1, 1:1
Volume of reservoir (µl)	500