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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Nat Struct Mol Biol. 2012 Sep 24;19(11):1116–1123. doi: 10.1038/nsmb.2412

Figure 2.

Figure 2

APC15 depletion causes delay in mitosis. (a) Still images of time-lapse microscopy experiments showing representative cells at nuclear envelope breakdown (NEBD), metaphase, anaphase and telophase (from left to right). Time of progression for control or APC15 RNAi treated cells after NEBD (h:min) is indicated for the respective cell cycle stages. Size bar equals 5 μm. H2B-mCherry shown in magenta. β-tubulin shown in green. (b) Histogram showing quantification of time-lapse microscopy experiments. Average time of progression of control or APC15 RNAi treated cells from NEBD to anaphase and anaphase to cytokinesis (n=25; error bars denote s.e.m.; asterisks denote P-value of 0.0005 from a two-tailed t test). (c) Histogram showing quantification of mitotic stages after control or APC15 RNAi treatment (n>8600 per condition). CDC20, BUB1, α-tubulin and DNA staining were used to distinguish different mitotic stages. (d) Histogram showing quantification of mitotic index after control RNAi or APC15 or/and MAD2 RNAi (n=5000 per condition). Phospho-H3Ser10, α-tubulin and DNA staining were used to distinguish mitotic and interphase cells.