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. 2012 Jul 1;2(3):151–160. doi: 10.4161/cl.20490

Table 1. Lipid-signaling and -metabolism proteins required for yeast polarized exocytosis.

Protein Activity Localization
Actinomyosin-based vesicle motility
 
 
Myo2
Type V myosin motor
Actin filaments
Exocyst complex/vesicle tethering
 
 
Sec3, Exo70
PM-associated exocyst complex subunits
PM/sites of polarized growth
Exo84, Sec6, Sec8, Sec10, Sec15
Vesicle-associated exocyst complex subunits
Exocytic vesicles/sites of polarized growth
Lipid flippases and regulators
 
 
Cdc50
Drs2p chaperone
Golgi
Dnf1, Dnf2
Aminophospholipid translocases
PM
Dnf3, Drs2
Aminophospholipid translocases
Golgi
Lem3
Dnf1p interacting protein
PM
Markers for exocytic transport
 
 
Can1
Plasma membrane arginine permease
PM
Fur4
Uracil permease
PM
Gas1
Beta-1,3-glucanosyltransferase
PM, ER, Golgi
Pma1
Plasma membrane H+-ATPase
PM
Tat2
High-affinity tryptophan and tyrosine permease
PM
Phospholipid metabolism and regulators
 
 
Cho1
PS synthase
Microsomes
Frq1
Pik1p regulator
Golgi
Osh4/Kes1
Oxysterol-binding protein homolog; Sac1p regulator
Golgi, exocytic vesicles, cytoplasm, endosomes
Mss4
PI-4P 5-kinase
PM
Pct1
Cholinephosphate cytidylyltransferase
Golgi
Pik1
PI 4-kinase
ER, Golgi
Pis1
PI synthase
Golgi
Sac1
PI 4-P phosphatase
ER, Golgi
Sec14
PI/PC transfer protein
Golgi
Sph5
PI transfer protein
Cytoplasm, ER, PM
Spo14
Phospholipase D
Endosomes
Stt4
PI 4-kinase
PM
Rab GTPases and regulators
 
 
Gdi1
Rab GDI (GTPase dissociation inhibitor)
Cytoplasm
Mrs6
Rab GEP (GTPase escort protein)
Cytoplasm
Sec2
Sec4p GEF (guanine nucleotide exchange factor)
Golgi, exocytic vesicles, sites of polarized growth
Sec4
Rab-family GTPase
Golgi, exocytic vesicles, sites of polarized growth
Yip1
Rab GTPase- and GDI-interacting protein
ER, Golgi and COPII vesicle membranes
Ypt31, Ypt32
Rab-family GTPases
Endosome, Golgi
Ras GTPases and regulators
 
 
Age1, Age2
ARF GAPs
Golgi
Arf1
Ras-family GTPase
Golgi-associated vesicles
Gcs1
ARF GAP
Golgi
Sec7
ARF GEF
Cytoplasm, Golgi, Golgi-associated vesicles,
Rho GTPases and regulators
 
 
Cdc24
Cdc42p GEF
PM/sites of polarized growth, nucleus
Cdc42
Rho-family GTPase
PM/sites of polarized growth
Gic2
Cdc42p effector
PM
Rdi1
Rho GDI
Cytoplasm, PM/sites of polarized growth
Rga1, Rga2
Cdc42p GAPs
PM/Bud neck
Rho1
Rho-family GTPase
Golgi, PM/sites of polarized growth
Rho3
Rho-family GTPase
PM/sites of polarized growth
Rom2
Rho1p and Rho2p GEF
PM/sites of polarized growth
SNARE membrane fusion complex
 
 
Sec9
t-SNARE, SNAP-25 homolog
PM
Snc1, Snc2
v-SNARE, synaptobrevin homolog
Exocytic vesicles, Golgi
Sso1, Sso2 t-SNARE, syntaxin homolog PM