Table 3. The 10 most different fold changes miRNAs between peak and late lactation.
miRNAs | Reads | Nor_ Reads | F_ change | P_ Value | Sig_ level | ||
Peak lactation | Late lactation | Peak lactation | Late lactation | ||||
miR-17a* | 0 | 54,914 | 0.01 | 7,435 | 19.50 | 0 | ** |
miR-5425 | 0 | 6,766 | 0.01 | 916 | 16.48 | 0 | ** |
miR-4754 | 0 | 6,326 | 0.01 | 856 | 16.37 | 0 | ** |
miR-3138 | 10,637 | 0 | 559 | 0.01 | −15.77 | 0 | ** |
miR-125a* | 15,723 | 0 | 826 | 0.01 | −16.33 | 0 | ** |
miR-125* | 20,053 | 0 | 1,052 | 0.01 | −16.68 | 0 | ** |
miR-29-3p | 21,450 | 0 | 1,126 | 0.01 | −16.78 | 0 | ** |
miR-3966 | 25,136 | 0 | 1,320 | 0.01 | −17.01 | 0 | ** |
miR-3175 | 27,377 | 0 | 1,438 | 0.01 | −17.13 | 0 | ** |
miR-881 | 29,370 | 0 | 1,542 | 0.01 | −17.23 | 0 | ** |
Note: Nor_Reads: Normalized reads, the results of Solexa sequencing, Normalization formula: Normalized expression = Actual miRNA count/Total count of clean reads×1,000,000; F_change: Fold_changes (Log2 Late lactation/Peak lactation), fold changes of miRNAs in both samples, – represents down regulation in late lactation; P_Value: P values manifest the significance of miRNAs differential expression between two samples; Sig_level: Significance_level, # represents no significant difference, * represents significant difference;