Table 4. Summary of miRNA primers used in real-time RT-PCR.
| Primer | Sequences (5′→3′) | Length (nt) | GC (%) | Tm |
| U6 | CAAGGATGACACGCAAATTCG | 21 | 47.6 | 69 |
| bta-miR-15b | CGTAGCAGCACATCATGGTTTACA | 24 | 45.8 | 72 |
| bta-miR-107 | GAGCAGCATTGTACAGGGCTATC | 23 | 52.2 | 73 |
| bta-miR-30b-5p | GCCGAAACATCCTACACTCAGCT | 23 | 52.2 | 73 |
| bta-miR-214 | AGCAGGCACAGACAGGCAGT | 20 | 60.0 | 72 |
| bta-miR-193a-5p | GTCTTTGCGGGCGAGATGA | 19 | 57.9 | 69 |
| bta-miR-339b | CCATATCTGTCCTCCAGGAGCTC | 23 | 56.5 | 75 |
| bta-miR-375 | TTCGTTCGGCTCGCGTGA | 18 | 61.1 | 69 |
| bta-miR-487b | GCAATCGTACAGGGTCATCCAC | 22 | 54.5 | 70 |
| bta-miR-339b | CCATATCTGTCCTCCAGGAGCTC | 23 | 56.5 | 75 |
| bta-miR-100 | AACCCGTAGATCCGAACTTGTG | 22 | 50.0 | 71 |
| Novel_3 | TACGAGGACCTCCAGAACGACC | 22 | 59.1 | 65 |
| Novel_5 | CCAGGCTAGGAGAAATGATTGG | 22 | 50.0 | 62 |
| Novel_7 | CCCAAACCAGTTGTGCCTGTAG | 22 | 54.6 | 64 |
| Novel_10 | AGCGCAAATGAACGTTTTGG | 20 | 45.0 | 63 |
| Novel_16 | CCAGGGATGTAGCTCCTAGTGC | 22 | 59.1 | 63 |
| Novel_24 | TGGGAGTCGCTGAGAGGATGGA | 22 | 60.9 | 69 |
| Novel_25 | CAGGGAGAAGGTGAGCAGAGGA | 22 | 59.1 | 65 |
| Novel_27 | TGTGAGAGAAGAGGACGCCTGG | 22 | 59.1 | 66 |
| Novel_32 | ATATATCCAGGGGACGCCGTT | 21 | 52.4 | 64 |
| Novel_33 | CCGCCGGTAAAAAATTGATTTGACT | 25 | 40.0 | 67 |
| Novel_34 | GCGTCAAGTGATGGAGAAGATGA | 23 | 47.8 | 64 |
| Novel_35 | AATTCCGGTCGCTGTGCTCTCG | 22 | 59.1 | 70 |
| Novel_37 | GCGAACCTGAGCAAACTTTTT | 21 | 42.9 | 61 |
| Novel_38 | CACTGATTGGGGTAGATGTGAGA | 23 | 47.8 | 62 |
| Novel_39 | CAGCAACTAAAGATCCCTCAGG | 22 | 50.0 | 60 |
| Novel_41 | ATAGAGAGGGATGGGCTGGG | 20 | 60.0 | 63 |
| Novel_42 | CGCTGAACAAACTTTTTGGCCA | 22 | 45.5 | 66 |
| Novel_43 | TGCCAAGCCCACGTTCAAAGG | 21 | 57.1 | 70 |
| Novel_44 | TGGGGAAGCGCAGGAAACAGT | 21 | 57.1 | 68 |
| Novel_45 | TGAGTTAAAGGAAAAGGAAAGT | 22 | 31.8 | 54 |
| Novel_46 | AATGTACTTGTGGAGTTGGAG | 21 | 42.9 | 54 |
| Novel_48 | TGGACAACTGAAGCTAGCACAG | 22 | 50.0 | 61 |
| Novel_50 | ATAGAGCCCCGGTAGACTGT | 20 | 55.0 | 58 |