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. 2012 Nov 14;7(11):e49495. doi: 10.1371/journal.pone.0049495

Figure 8. Assays for the activation of FgHog1, Mgv1, and Gpmk1 MAP kinases.

Figure 8

Total proteins were isolated from vegetative hyphae of the wild type (PH-1) and the Fghog1 (HG15), Fgpbs2 (PS15), and Fgssk2 (FK13) mutants. A. The anti-TpGY antibody was used to detect the phosphorylation of FgHog1 (41-kDa) in cultures treated with or without 0.7 M NaCl. B. Phosphorylation of Mgv1 (46-kDa) and Gpmk1 (42-kDa) was detected with the anti-TpEY antibody. Anti-actin and anti-MAPK antibodies were used to determine the same loading amount of protein.