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. 2012 Nov 14;7(11):e49530. doi: 10.1371/journal.pone.0049530

Figure 1. Extract of AMK inhibits adipocyte differentiation of 3T3-L1 cells.

Figure 1

Two-day post-confluent 3T3-L1 preadipocytes (day 0) were treated with the indicated concentrations of aristolochia manshuriensis Kom extract and was repleted every 2 days along with relevant media cocktail up to day 8. Cells treated with 1X PBS were used as control. (A) Cell viability was determined by MTT assay. (B) Intracellular lipids were stained Oil-Red O. (C) Absorbance was spectrophotometrically determined at 500 nm after Oil-Red O staining. (D) Triglyceride (TG) content (per mg protein) was measured with a TG-S reaction kit (Asan Pharm. Co., Seoul, Korea). Three biological replicates of aristolochia manshuriensis Kom extract-treated adipocytes were tested. The results were confirmed by three independent experiments, which were each conducted in triplicate. Data are expressed as the mean ± S.D. **P<0.01 vs. controls.