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. 2012 Nov 14;7(11):e49530. doi: 10.1371/journal.pone.0049530

Figure 5. AA and fractions of AMK constitutes inhibits adipocyte differentiation of 3T3-L1 cells.

Figure 5

Two-day post-confluent 3T3-L1 preadipocytes (day 0) were treated with the indicated concentrations of AA and fractions of AMK constitutes such as EtOAc fraction with AA and BuOH fraction without AA, and was repleted every 2 days along with relevant media cocktail up to day 8. Cells treated with 1X PBS were used as control. (A) Cell viability was treated with fractions of AMK constitutes (EtOAc and BuOH) and (B) AA was determined by MTT assay. Intracellular lipids were stained Oil-Red O. (C) Absorbance was spectrophotometrically determined at 500 nm after Oil-Red O staining (C) treatment of BuOH fraction; (D) treatment of EtOAc fraction; (E) treatment of AA. The results were confirmed by three independent experiments, which were each conducted in triplicate. Data are expressed as the mean ± S.D. **P<0.01 vs. controls.