Figure 6. AA and fractions of AMK constitutes inhibits the differentiation of 3T3-L1 cells by regulation of Ras/Raf/MEK/ERK1/2 phosphorylation and PDK/Akt phosphorylation.

Preadipocytes were induced to differentiate with AA (20 µmol), EtOAc fraction (20 µg/mL), BuOH fraction (200 µg/mL), and harvest at 30 min, 1 h and 2 h and during the early-stage of differentiation. (A) The phosphorylation of ERK1/2 and Akt. (B) The expression of Ras, pRaf1, pMEK1, and pPDK1. The proteins were analyzed by western blot. Results were expressed relative to untreated cells after normalization to β-actin mRNA. Values are mean ± S.D. of data from three separate experiments; each experiment was performed in triplicate.