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. 2012 Nov 14;7(11):e49260. doi: 10.1371/journal.pone.0049260

Figure 1. C4B6− cells are small, round cells which are abundant among the PBL.

Figure 1

(A) Double immunostaining with anti-TO (green) and MAb C4B6 (red) revealed small, round cells that were C4B6− (white arrows). (B) Flow cytometry histograms of PBL prior and after MACS separation. The bound fraction (C4B6+ cells) contained both polymorphonuclear (PML) and mononuclear cells (L = leukocytes, M = monocytes) while the cells in the unbound fraction (C4B6− cells) were small, mononuclear cells with low level of granularity. Cells with large, round nuclei were present in most C4B6− cells (labeled with *), although cells with other nuclei forms were observed. C4B6+ cells are shown as red dots in the dot plots (middle panels). The markers define the C4B6+ cells. Diff Quick stained cells are shown in the right panels. Representative single cells are enlarged. The diff quick pictures are captured with 63× objective. Scale bar = 5 µm.