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. 2012 Nov 14;7(11):e49260. doi: 10.1371/journal.pone.0049260

Figure 4. The C4B6− cells have an oxygen-independent mechanism.

Figure 4

Panels to the left, C4B6+ cells, are included as positive controls. Panels to the right, C4B6− cells. (A) Respiratory burst activity. Cells were stimulated with PMA and positive cells appear green in fluorescence microscopy due to RHO123 fluorescent (left panels). The corresponding bright field photos are shown in the right panels. Magnification: 400×. (B) Myeloperoxidase activity. Positive cells appear brown while cells without activity are blue. (C) Acid phosphatase staining. Granular sites of activity appear red. For the C4B6− cells, notice that the overview picture to the right has less counterstaining, for easier observation of the positive granules. (D) Tartrate resistant acid phosphatase staining. Granular sites of activity appear red. Representative single cells are enlarged; PMN = polymorphonuclear, M = monocytes, L = leukocytes,* = representative C4B6− cells, T = thrombocytes. Pictures in B–D are captured with 63× objective. Scale bar = 10 µm.