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. 2012 Nov 14;7(11):e49374. doi: 10.1371/journal.pone.0049374

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© 2012 Nespital, Strous

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Mouse 3T3-F442A preadipocytes were incubated at 37 or 40°C for 4 h and then treated with the indicated concentrations of GH for 10 min. The cells were lysed in 1% SDS and equal amounts of lysate were analyzed by western blotting (WB), using anti-Jak2, anti STAT5b and anti-actin antibodies. (B) Quantification of Fig. 2A. At each point the ratio of the lower band to the total STAT5b signal was calculated (100 ng/ml GH at 37°C was set 100%; 0 ng/ml was set 0%). The data represent the mean of four independent experiments SEM. (circles, 37°C; squares, 40°C).

Inline graphic — (A) Mouse 3T3-F442A preadipocytes were incubated at 37 or 40°C for 4 h and then treated with the indicated concentrations of GH for 10 min. The cells were lysed in 1% SDS and equal amounts of lysate were analyzed by western blotting (WB), using anti-Jak2, anti STAT5b and anti-actin antibodies. (B) Quantification of Fig. 2A. At each point the ratio of the lower band to the total STAT5b signal was calculated (100 ng/ml GH at 37°C was set 100%; 0 ng/ml was set 0%). The data represent the mean of four independent experiments SEM. (circles, 37°C; squares, 40°C).