Figure 4. Responses of EcR-B1 reporters to 20E in M1 and Sf9 cells.

M1 and Sf9 cells were transfected with representative EcR-B1 reporter plasmids (see Fig. 3), treated with 1 µM of 20E during 3 days, and a dual reporter assay was conducted. −3652/+248, the full length reporter construct; mu02, an E1 region mutant; mu05, a mutant within the internal region between E1 and E2; mu06, an E2 region mutant. Bar represent SE (N = 4).