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. 2012 Nov 14;7(11):e49372. doi: 10.1371/journal.pone.0049372

Figure 3. PCR amplification of Ubi4 and Ubi10 from various templates.

Figure 3

(A) Primers used for amplification of Ubi4 (Ubi4Fw and Ubi4Rv) and Ubi10 (Ubi10Fw and Ubi10Rv) are according to Hong et al. (2008) [5]. Lane 1: molecular weight marker (Bioline HyperLadder 1). The templates used are indicated for each lane. Empty vector (pGEM-T Easy) and no RT reactions were included as controls. The results shown are reverse gel images (dark bands on white backgrounds) from 25 amplification cycles. (B) Primers targeting the 3′-UTRs of Ubi4 (Ubi4-3Fw and Ubi4-3Rv) and Ubi10 (Ubi10-3Fw and Ubi10-3Rv) were used for amplification of the various templates. Lane assignments and templates used as in (A). Gel images are representative of 3 independent experiments.