Figure 3. Overexpression of C-terminally myc-tagged Hch1p impairs growth of yeast expressing Hsp82p^A587T but not yeast expressing wildtype Hsp82p.

A. Cells were grown overnight in YPD supplemented with 200 mg/L G418 and then diluted to 1×10⁸ cells per mL. 10-fold serial dilutions were prepared and 5 µL aliquots were spotted on YPD agar plates supplemented with 200 mg/L G418 and grown for 2 days at 30, 35.5 and 37°C. B. Western analysis of yeast shown in 3A with anti-myc tag, anti-His tag and anti-actin antibodies. Levels of mycHch1p (lanes 2, 4, 6, and 8) in strains expressing Hsp82p (lanes 1–4), or Hsp82p^A587T (lanes 5–8) with (lanes 3,4, 7, 8) or without (lanes 1, 2, 5, 6) HCH1 deletion. (Anti-myc antibody 9E10).