Figure 3. The levels of some key myeloid markers are altered in the brain of IRF8-deficient mice.

Immunostaining was performed on brain sections from healthy, adult WT (A–D) or IRF8-deficient (E–H) mice as described in the Materials and Methods. Panels A, B, C, E, F, G show cortex while panels D, H show cerebellum (original magnification all panels 1000X). For immunofluorescence (G, H) DAPI was used to stain nuclei. Whole brain lysates were prepared from healthy, adult mice and 20 µg of protein analysed by western blotting (I).