Figure 4. GFP⁺ cells in the CNS are exclusively CD11b⁺ microglia.

Flow cytometry was performed on cells isolated from the brain of healthy adult WT (A, B) and IRF8-deficient mice as described in the Materials and Methods. For analysis, cells were scatter-gated to exclude dead cells and GFP⁺ cells were selected (A). Greater than 99% of GFP⁺ cells expressed CD11b in WT and IRF8-deficient mice (B), consistent with a microglial phenotype. Isotype matched antibodies were used to determine background staining (data not shown).