Figure 5. Surface levels of various molecules are altered on IRF8-deficient microglia.

Flow cytometry (A-J) was performed on microglial cells isolated from the brain of adult WT (black dashed line) and IRF8-deficient (black line) mice as described in the Materials and Methods. For analysis, cells were scatter-gated to exclude dead cells and were selected for GFP, CD11b expression. An isotype matched antibody was used as a negative control (grey dashed line). Quantification of forward and sideward scatter; (K) or mean fluorescent intensity of histograms (L). The histograms represent means +/− SD from three separate experiments. For significance: ***P<0.001, **P<0.01, *P<0.05; by two-tailed t-test.