Figure 6. IRF8-deficient microglia show no defect in E.coli particle phagocytosis.

Flow cytometry analysing pHrodo fluorescent E.coli particle uptake after 3 h by WT (A) and IRF8-deficient (B) primary microglia (grey dashed line = 4°C control assay, black continuous line = 37°C). Cells were gated for eGFP and CD11b (CD11b-PerCP-Cy5.5) and pHrodo fluorescent E.coli particle uptake was determined by measuring signal increase in the red fluorescence channel (fl2).