Table 1. Survey of tested target sites.
| Identity: pUC19- | Length1 | Sequence2 | Cleavage efficiency3 |
| A | -12/+24 | CAGTTAGACGGGAAGACCCTATGCAGCTTTACTGTA | +++ |
| B | −24/+24 | GCGGTTTACCTTCAGTTAGACGGGAAGACCCTATGCAGCTTTACTGTA | +++ |
| C | −24/+12 | GCGGTTTACCTTCAGTTAGACGGGAAGACCCTATGC | +++ |
| D | −12/+12 | CAGTTAGACGGGAAGACCCTATGC | +++ |
| E | −24/+24 | GCGGTTTACCTTCAGTTAGACGCTCTGACCCTATGCAGCTTTACTGTA | – |
| F | −12/+12 | CAGTTAGACGCGAAGACCCTATGC | ++/+++ |
| G | −11/+11 | AGTTAGACGGGAAGACCCTATG | ++/+++ |
| H | −9/+9 | TTAGACGGGAAGACCCTA | ++/+++ |
| I | −7/+7 | AGACGGGAAGACCC | +/− |
| K | −12/+12 | CAGTTAGACGAGAAGACCCTATGC | ++/+++ |
| L | −12/+12 | CAGTTAGACGGGCTGACCCTATGC | +/− |
| M | −12/+12 | CAGTTATACGGGAAGACCCTATGC | ++/+++ |
| N | −8/+8 | TAGACGGGAAGACCCT | ++/+++ |
| O | −12/+12 | CAGTTAGACGCTAAGACCCTATGC | ++/+++ |
| P | −7/+9 | AGACGGGAAGACCCTA | ++/+++ |
| Q | −9/+7 | TTAGACGGGAAGACCC | +/− |
| R | −6/+9 | GACGGGAAGACCCTA | ++/+++ |
| T | −12/+12 | CAGTTAGACGGGCAGACCCTATGC | ++ |
| U | −12/+12 | CAGTTAGACGGGATGACCCTATGC | ++ |
| V | −12/+12 | CAGTTAGACGGGAACACCCTATGC | +++ |
| W | −12/+12 | CAGTTAGACTGGAAGACCCTATGC | ++/+++ |
| X | −12/+12 | CAGTTAGACTCGAAGACCCTATGC | + |
| Y | −6/+8 | GACGGGAAGACCCT | + |
| Y* | −6/+8 | GACGGGCAGACCCT | – |
| Z | −5/+9 | ACGGGAAGACCCTA | – |
1
The numbering refers to the central-four base pairs GG−1A+1A.
2
The central-four nucleotides (or mutated derivatives) of the cleavage site are in bold face type. Nucleotide substitutions are underlined and in bold face type.
3
See legend of Figure 3 for the assignment of + and - symbols.